Exercises for Lesson 2
1. List the three parts of the Vacutainer® system.
2. List the steps required to obtain a blood specimen with a Vacutainer®.
(Begin after the Vacutainer® and needle have been assembled.)
3. Describe the steps to follow to position and insert a needle in a vein to
obtain a blood sample.
4. What items must be included on the Laboratory Request slip?
5. List the steps to follow to obtain a blood sample with an assembled
Solutions to Exercises for Lesson 2
- Double-ended needle,
- Plastic holder
- Vacuum tubes marked with a guideline (para 2-1a)
- Expose area for venipuncture.
- Select vein.
- Position protective pad.
- Prepare pledget (sponge) for use.
- Apply constricting band.
- Palpate selected vein.
- Cleanse the skin.
- Position needle.
- Puncture vein.
- Collect specimen.
- Withdraw the needle.
- Remove specimen tube from vacutainer.
- Apply self-adhesive bandage.
- Provide for patient's safety and comfort.
- Dispose of and/or store equipment.
- Perform patient care handwash.
- Position the needle directly in line with the vein and grasp the patient's
arm below the point of entry with the free hand.
- Place the thumb approximately one-inch below the expected point of entry and
pull the skin taut and toward the hand.
- Place the needle bevel up in line with the vein and pierce the skin
approximately at a 15 to 45 degree angle.
- Decrease the angle until almost parallel to the skin surface and direct it
towards the vein.
- Pierce the vein wall.
4. The Laboratory Request form should contain:
- Complete patient identification.
- Requesting physician's signature.
- Ward number, clinic, or dispensary designation.
- Date and time of specimen
- Test(s) requested.
- "URGENCY" box completed.
5. Syringe volume should not exceed 20 cc.
- Place constricting band and penetrate vein as with Vacutainer. Pull plunger
- Filled syringe may be removed from needle and a new one attached.
- Insert the needle into the rubber stopper of the tube to transfer the
specimen into the tube.